Beijing said it has deep concerns over the EU's Emissions Trading Scheme (ETS), which came into force on New year's Day and demands all airlines pay a green duty to offset carbon emissions.
"China opposes the European Union's unilateral legislation. China has expressed to the EU our deep concern and opposition many times on a bilateral level," Chinese Foreign Ministry spokesman Hong Lei said.
Mr Hong urged Brussels to hold urgent talks with Beijing over the controversial carbon allowance scheme, which has also met strong opposition from other countries.
The China Air Transport Association was more militant in its response – declaring its members would not co-operate with the ETS and refuse to pay the added tax.
It also said it would seek legal action and try and attempt to form an international alliance to scrap the scheme.